Secondary processes in atmospheric pressure chemical ionization-ion trap mass spectrometry: a case study of orotic acid |
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Authors: | Fryčák Petr Jirkovský Jaromír Ranc Václav Bednář Petr Havlíček Vladimír Lemr Karel |
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Institution: | Regional Centre of Advanced Technologies and Materials, Department of Analytical Chemistry, Faculty of Science, Palacky University in Olomouc, Olomouc, Czech Republic. |
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Abstract: | Atmospheric pressure chemical ionization is known for producing unusual artifacts of the ionization process in some cases. In this work, processes occuring in atmospheric pressure chemical ionization/MS of orotic acid that afforded ions accompanying protonated and deprotonated orotic acid molecules in the spectra were studied. Two processes ran in parallel in the ion source: decarboxylation of neutral orotic acid and collision-induced dissociation of its protonated or deprotonated form. A procedure discerning pre-ionization decomposition and post-ionization dissociation by manipulating ion source parameters was proposed. Experiments with isotopically labeled solvents confirmed ion-molecule reactions of the product of collision-induced dissociation of protonated orotic acid with solvent molecules in the ion source and even under vacuum in the ion trap. |
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