硫胺素-三维荧光法测定水中汞离子的研究

汞是一种具有显著积累效应和遗传毒性的重金属元素,对人体健康和生态环境危害极大.我国水环境中汞污染严重,开发快速、高效、经济的汞离子检测方法可以有效推动水环境中汞污染的源头治理.该研究创新性地提出利用硫胺素-三维荧光法来实现水环境中汞离子的检测.研究结果表明,硫胺素与汞离子发生氧化还原反应前后,其荧光峰的位置与数量发生了...

Study on Measurement of Mercury Ion in Water by Thiamine-Fluorescence Excitation-Emission Matrix

Mercury is a heavy metal element with significant accumulation effect and genotoxicity, which is extremely harmful to human health and the ecological environment. In China, the water environment is facing serious problems of mercury pollution. Developing rapid, efficient, and economical method for mercury ion detection can effectively promote the source control of mercury pollution in the aquatic environment. This study innovatively proposed a method, namely thiamine-fluorescence excitation-emission matrix (EEM), to monitor mercury ion in the water environment. Results showed that the position and number of fluorescence peaks of thiamine significantly changed after it happed redox reaction with mercury ion, which could be used as a characteristic signal for detecting mercury ions in water. In addition, when using this method to detect mercury ions in water, it was suggested that the concentration of thiamine should not be too high, and the reaction system should be kept in the alkaline environment. The reaction temperature and reaction time could be further optimized by the first-order kinetic model to reduce the detection cost and improve the detection effectiveness. Under the specific detection conditions (thiamine concentration 10 μmol·L-1, pH 9.7, reaction time 120 min, temperature 20 ℃),the linear detection range of mercury ion concentration was suggested to 4～15 μmol·L-1. The thiamine-EEM method owns outstanding advantages and good practical application values compared with the traditional method of mercury ion monitoring in water, which can effectively help the pollution source supervision of mercury in water environment and greatly improve the efficiency of environmental law enforcement.