Examination of some reversed-phase high-performance liquid chromatography systems for the determination of lipophilicity |
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Authors: | D. P. Nowotnik T. Feld A. D. Nunn |
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Affiliation: | Bristol-Myers Squibb Pharmaceutical Research Institute, 1 Squibb Drive, P.O. Box 191, New Brunswick, NJ 08903-0191 USA |
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Abstract: | Three reversed-phase systems [based on the divinylbenzene-styrene copolymer (PRP-1), the C18-derivatized divinylbenzene-styrene copolymer (ACT-1), and the Nucleosil C8 columns] were studied for their suitability in lipophilicity determination. Acetonitrile-water was selected as the mobile phase. Correlation between log k′ and log Pcyc for both the PRP-1 and Nucleosil C8 systems was superior to the correlation between log k′ and either log Poct or log Pcyc (oct = octanol; CYC = cyclohexane) on the ACT-1 column. On the PRP-1 and Nucleosil columns, correlation between log k′ and log Poct was much improved when test compounds were grouped into classifications of non-H bonding, single amphiprotics (alcohols, phenols, amides) or double amphiproties. Although the PRP-1 system gave broad peaks with lipophilic substrates, there was good correlation between log k′ values on the Nucleosil silica-based reversed-phase system and the polymer PRP-1 system, indicating that either is suitable for the determination of lipophilicity. |
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