| 辽东楤木叶中皂苷Congmuyenoside B(2)的分离与鉴定 |
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| 引用本文： | 张雷,陈燕萍,王志才,马兴元,杨帆,徐芳菲. 辽东楤木叶中皂苷Congmuyenoside B(2)的分离与鉴定[J]. 分析化学, 2007, 35(8): 1221-1224 |
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| 作者姓名： | 张雷 陈燕萍 王志才 马兴元 杨帆 徐芳菲 |
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| 作者单位： | 吉林大学化学学院,长春,130021;中国科学院长春应用化学研究所,长春,130022;集安益盛药业有限公司,集安,134200 |
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| 基金项目： | 吉林省科技发展计划项目(No990302) |
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| 摘 要： | 本研究实现了对辽东楤木叶中Congmuyenoside B(2)的分离与鉴定。研究中采用大孔吸附树脂提取法和硅胶柱层析法对其分离,应用HPLC色谱法和薄层色谱法对纯度进行分析,利用电喷雾多级串联质谱结合核磁共振和红外光谱对该化合物的结构进行分析鉴定。本提取分离法操作简便,提高了分离化合物的纯度。HPLC色谱法灵敏度高,结果准确、减少了质谱及其它色谱中的杂质峰对化合物鉴定的干扰,结构准确,可靠。
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| 关 键 词： | 辽东楤木叶 Congmuyenoside B(2) 高效液相色谱 电喷雾串联质谱 |
| 修稿时间： | 2006-12-14 |
Separation and Identification of Congmuyenoside B(2)From Leaves of Aralias Elate |
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| Zhang Lei,Chen Yan-Ping,Wang Zhi-Cai,Ma Xing-Yuan,Yang Fan,Xu Fang-Fei. Separation and Identification of Congmuyenoside B(2)From Leaves of Aralias Elate[J]. Chinese Journal of Analytical Chemistry, 2007, 35(8): 1221-1224 |
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| Authors: | Zhang Lei Chen Yan-Ping Wang Zhi-Cai Ma Xing-Yuan Yang Fan Xu Fang-Fei |
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| Abstract: | The saponin was isolated and characterized from leaves of Aralia elata.The large pore adsorption resin and silica gel column chromatographic method were used to extract and separate the saponin.HPLC and TLC were used to analyze the purity.The electrospray tandem mass spectrometry,combined with IR and NMR,was used for the structure determination.The compound was identified as Congmuyenoside B(2).The purification method is simple.and the HPLC method has the advantage of accuracy and high sensitivity,and the interference of impurity on the MS and other characterization method was reduced. |
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| Keywords: | Leaves of Aralia elata congmuyenoside B(2) high performance liquid chromatography electrospray tandem mass spectrometry |
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