Cu(II)、Ni(II)配合物的合成、抗肿瘤作用及其对细胞周期分布的影响

以2-氨基-5-氯苯酚和2-喹啉甲醛合成的席夫碱作为配体,分别与氯化镍、氯化铜反应合成了2个金属配合物C1和C2,其结构通过单晶X-射线衍射进行了解析。采用MTT法测试了配合物C1和C2对不同人肝癌细胞系和正常肝细胞系HL-7702增殖抑制活性,结果表明C1、C2对人肝癌细胞系的抑制活性均优于顺铂,且对正常肝细胞系HL-7702的毒性要弱于顺铂。通过活性氧实验、细胞周期等实验,可以推断出配合物C1、C2抗肿瘤机制是通过产生活性氧造成肿瘤细胞的氧化损伤,以及使细胞周期停滞在G0/G1期阻滞细胞复制。

Cu (II) and Ni (II) Complexes: Synthesis, Antitumor Effects and Cell Cycle Arrest

Two metal complexes, named C1 and C2, were synthesized by reaction of Schiff base synthesized from 2-amino-5-chlorophenol and 2-quinoline formaldehyde with nickel chloride and copper chloride respectively. Their structures were characterized by single crystal X-ray diffraction. The inhibitory activity of complexes C1 and C2 on the proliferation of different human hepatoma cell lines and normal hepatoma cell lines HL-7702 was tested by MTT method. The results showed that the inhibitory activity of C1 and C2 on human hepatoma cell lines was better than that of cisplatin, and the toxicity of C1 and C2 on normal hepatoma cell lines HL-7702 was weaker than that of cisplatin. Through the experiments of reactive oxygen species and cell cycle, it can be concluded that the anti-tumor mechanism of complexes C1 and C2 is the oxidative damage of tumor cells caused by the production of living oxygen and the arrest of cell cycle in G0 / G1 phase.