Gold nanoparticles as spectroscopic enhancers for in vitro studies on single viruses |
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Authors: | Dragnea Bogdan Chen Chao Kwak Eun-Soo Stein Barry Kao C Cheng |
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Institution: | Department of Chemistry and Department of Biology, Indiana University, Bloomington, Indiana 47405, USA. dragnea@indiana.edu |
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Abstract: | Gold particles with diameters between 2.5 and 4.5 nm have been introduced in the inner cavity of an icosahedral brome mosaic virus. The optical properties of single gold-marked virions have been tested in-vitro with respect to the characteristic plasmon polariton resonance. The shift in the plasmon polariton resonance of a single Au particle encapsulated in a virus with respect to a free particle in solution indicates a close interaction between the basic residues on the inner wall of the capsid and the negative surface charge of the particle. Incorporation of a pair of Au particles, approximately 4 nm diameter, is shown to be a frequent event. In this case, the dependence of the two-particle surface plasmon spectrum on the interparticle distance and the strong particle/capsid wall interaction suggest that, in the future, it will be possible to use encapsulated Au particles to track changes in the viral capsid volume in real-time and in a physiological environment. |
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