Interactions of Cu2+ ions with chicken prion tandem repeats |
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Authors: | Stańczak Pawel Łuczkowski Marek Juszczyk Paulina Grzonka Zbigniew Kozłowski Henryk |
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Affiliation: | Faculty of Chemistry, University of Wroc?aw, F. Joliot-Curie 14, 50-383 Wroclaw, Poland. |
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Abstract: | The potentiometric and spectroscopic (EPR, UV-Vis, CD) data have shown that the chicken prion hexa-repeat (Ac-His-Asn-Pro-Gly-Tyr-Pro-NH(2)) is a very specific ligand for Cu(2+) ions. The His imidazole is an anchoring binding site, then the adjacent amide nitrogen coordinates as a second donor. The presence of Pro at position 3 induces binding of phenolate oxygen as a third donor atom. The tridentate coordination dominates around physiological pH. Similar to human octapeptide fragments, chicken tandem repeats exhibit a cooperative effect in binding Cu(2+) ions, although chicken peptides are much less effective in metal ion coordination. |
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