The Fluorescence Properties and Lifetime Study of G-quadruplexes Single- and Double-labeled with Pyrene |
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Authors: | Anna Dembska and Bernard Juskowiak |
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Institution: | (1) Department of Chemistry, A.Mickiewicz University, Grunwaldzka 6, 60-780 Poznan, Poland; |
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Abstract: | We report steady state fluorescence and lifetime emission studies of d(GGTTGGTGTGGTTGG) (TBA) and d(GGGTTAGGGTTAGGGTTAGGG)
(Htelom) oligonucleotides labeled with pyrene through a 3-aminopropyl linker. Such G-rich sequences are able to self-assemble
into G-quadruplexes, especially in the presence of specific cations like potassium. A comparative studies with single- and
double-labeled G-quadruplexes were carried out. For each probe we have measured fluorescence decays for emission wavelength
of 390 and 480 nm in the varying concentration of potassium ion. We have calculated average lifetimes <τ> for every system
as well as the fractional distribution αi of emitting species. |
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