Liquid chromatography with diode array detection and multivariate curve resolution for the selective and sensitive quantification of estrogens in natural waters |
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Authors: | Rocí o L. Pé rez,Graciela M. Escandar |
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Affiliation: | Instituto de Química Rosario (CONICET-UNR), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531 (2000), Rosario, Argentina |
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Abstract: | Following the green analytical chemistry principles, an efficient strategy involving second-order data provided by liquid chromatography (LC) with diode array detection (DAD) was applied for the simultaneous determination of estriol, 17β-estradiol, 17α-ethinylestradiol and estrone in natural water samples. After a simple pre-concentration step, LC–DAD matrix data were rapidly obtained (in less than 5 min) with a chromatographic system operating isocratically. Applying a second-order calibration algorithm based on multivariate curve resolution with alternating least-squares (MCR-ALS), successful resolution was achieved in the presence of sample constituents that strongly coelute with the analytes. The flexibility of this multivariate model allowed the quantification of the four estrogens in tap, mineral, underground and river water samples. Limits of detection in the range between 3 and 13 ng L−1, and relative prediction errors from 2 to 11% were achieved. |
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Keywords: | ANDR, Androgens BPA, Bisphenol A CF, Cigarette filter CORT, Corticosteroids CP-CPE, Co-precipitation assisted cloud point extraction DAD, Diode array detector DERIV, Derivatization DES, Diethylstilbestrol DHS, Dihydrostilbestrol DIS, Dienestrol DSW, Discharging sites water DLLME, Dispersive liquid&ndash liquid microextraction ECF, Estrogen conjugated forms ENNFM, Electrospun nylon6 nanofibrous membrane FD, Fluorescence detector FW, Fishpond water GC, Gas chromatography HF-MMLLE, Hollow-fiber microporous membrana liquid&ndash liquid extraction LC, Liquid chromatography LVI, Large volume injection LOD, Limit of detection LOQ, Limit of quantification LW, Lake water MES, Mestranol MIP, Molecularly imprinted polymer MM-SPE-MPS, Magnetic-mediated solid-phase extraction micro-particle sorbent MS, Mass spectrometry MS/MS, Tandem mass spectrometry MW, Mineral water OP, Octylphenol PPs, Pharmaceutical products PROG, Progestagens RW, River water SBSE, Stir bar sorptive extraction SPE, Solid-phase extraction SPW, Spring water SW, Surface water SWW, Sewage water TW, Tap water UPLC, Ultra performance liquid chromatography US, Ultrasonication UW, Underground water W, Water WW, Wastewater WWTPI, Wastewater treatment plant influent WWTPE, Wastewater treatment plant effluent |
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