LDH参与的机械-光信号转换体系的吸收光谱研究

用紫外-可见分光光度法研究了乳酸脱氢酶(LDH)参与的，以NADH与DPIP.，O₂为主体的机械-光信号振荡转换体系。LDH的酶催化作用使此振荡体系的信号转换效率大大提高，在无乳酸情况下，当DPIP.和NADH的摩尔比为1∶4.5时的平均循环周期由108 min缩短为34 min；在乳酸存在下，此体系的平均循环周期由108 min缩短为29 min。推测LDH的促进作用主要是通过对NADH的活化实现的，其次是通过酶促乳酸脱氢作用补充体系中的NADH获得的。结果说明，酶的催化作用在某些双底物之一存在的反应情况下，也会明显表现出来。

The Spectrum Studies of Mechano-Optical Signal Transduction with Participation of LDH

The optical response from a mechanical stimulus signal oscillating system of NADH,DPIP and O2 with the participant of lactate dehyrogenase(LDH) was studied with UV/Vis Spectrometer in the present paper.The signal transduction efficiency of the system was largely improved by the catalysis of LDH,when DPIP.:NADH was 1:4.5(mole ratio)without lactate,and the average period of system was shortened from 108 to 34 min,and to 29 min with lactate existing.It was presumed that the accelerating effect of LDH was mainly brought by the activation of NADH at its catalysis center,and in addition,brought by the supplement of NADH through the dehydrogenation of lactate.The results indicated that the catalysis of enzyme would be put up also when only one of the two substrates existed.