| 黑线仓鼠催乳素受体（PRLR）基因部分序列的克隆与序列分析 |
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| 引用本文： | 王东宽,董晓波,薛慧良,宛新荣,徐来祥.黑线仓鼠催乳素受体（PRLR）基因部分序列的克隆与序列分析[J].曲阜师范大学学报,2009,35(1):90-94. |
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| 作者姓名： | 王东宽 董晓波 薛慧良 宛新荣 徐来祥 |
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| 作者单位： | 王东宽,薛慧良,徐来祥,WANG Dong-kuang,XUE Hui-liang,XU Lai-xiang(曲阜师范大学生命科学学院,273165,山东省曲阜市);董晓波,DONG Xiao-bo(国家林业局森林病虫害防治总站,110034,辽宁省沈阳市);宛新荣,WANG Xin-rong(中国科学院动物研究所,100080,北京市)
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| 基金项目： | 国家自然科学基金，国家重点基础研究发展规划(973计划)，曲阜师范大学计划省级重点建设资助项目 |
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| 摘 要： | 采用基因克隆方法首次扩增出黑线仓鼠催乳素受体（Prolactin Receptor，PRLR）基因5’端部分序列（GenBank登录号为EU826030），测序结果表明该片段的有效长度为379bp，包含部分5’调控区、exonl及部分intron1．该序列与其它物种相应区域的同源性比较结果：黑线仓鼠与人、大鼠、小鼠、狐猿、黑猩猩、类人猿PRLR基因核苷酸序列的同源性为82．1％-93．4％；exon1序列的同源性为79．8％-91．4％．系统进化分析与物种亲缘关系的远近一致，同时证明PRLR基因exon1有较大的可变性，因此认为成功克隆了黑线仓鼠的PRLR基因的部分序列．该研究所得到的PRLR基因序列可用作研究物种亲缘关系或遗传距离的理想标记．
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| 关 键 词： | 黑线仓鼠 PRLR基因 克隆 序列分析 |
Cloning and Sequence Analysis on Partial Fragment of Prolactin Receptor Gene in Cricetulus Barabensis |
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| WANG Dong-kuang,DONG Xiao-bo,XUE Hui-liang,WANG Xin-rong,XU Lai-xiang.Cloning and Sequence Analysis on Partial Fragment of Prolactin Receptor Gene in Cricetulus Barabensis[J].Journal of Qufu Normal University(Natural Science),2009,35(1):90-94. |
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| Authors: | WANG Dong-kuang DONG Xiao-bo XUE Hui-liang WANG Xin-rong XU Lai-xiang |
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| Institution: | WANG Dong-kuang, DONG Xiao-bo, XUE Hui-liang, WANG Xin-rong, XU Lai-xiang (1.College of Lith Sciences,Qufu Normal University,273165, Qufu,Shandong; 2.General Station of Forest Pest Management, State Forestry Admnistration, 110034,Shenyang, Liaoning; 3.Institute of Zoology,The Chinese Academy of Sciences, 100080, Beijing, PRC) |
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| Abstract: | The partial sequence of 5＇end of prolactin receptor（PRLR） gene in Cricetulus barabensis was first cloned in this experiment, with Genbank submittion number EU826030. The 379 bp virtual fragment which contained partial sequence of 5＇end, the full length of exon 1 and partial intronlwas amplified successfully by PCR. The nucleotide homology of PRLR gene among Cricetulus barabensis ,Rattus norvegicus, Mus musculus, Homo sapiens, Pongo abelii, Macaca mulatta and Pan troglodytes was from 82.1% to 93.4%. The homology of exonl was from 79.8% to 91.4%. This result was similar to former reports, what＇s more, it could prove that the exonl was variable. So the partial fragment of Cricetulus barabensis was cloned successfully. To this content, it could be considered as an ideal marker for studying relationships among animals and genetic distance. |
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| Keywords: | Cricetulus barabensis PRLR cloning sequence analysis |
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