Visual observation of avidin-biotin affinity by fluorescent G4.5 poly(amidoamine) dendrimer |
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| Authors: | Saravanan Govindachetty Daigo Kenji Imae Toyoko Hamakubo Takao |
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| Affiliation: | Graduate School of Science and Technology, Keio University, Hiyoshi, Yokohama 223-8522, Japan. |
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| Abstract: | An air-treated G4.5 poly(amidoamine) (PAMAM) dendrimer displayed the enhanced fluorescence enough to be utilized as a fluorescence marker to visualize avidin-biotin affinity: On a fluorescence microscopic image, the avidin labeled by a fluorescent G4.5 PAMAM dendrimer was observed to be selectively bound on the biotin pattern that was prepared by amide-bonding of biotin on a carboxylic acid-terminated self-assembled monolayer and in turn by UV-irradiation with a photomask on the monolayer. |
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