A micromethod for the determination of selenium in tissues and biological fluids by single-test-tube fluorimetry |
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| Affiliation: | 1. Department of Pharmacy, Faculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, 21000 Novi Sad, Serbia;2. Department of Chemistry, Biochemistry and Environmental Protection, Faculty of Sciences, University of Novi Sad, Trg D. Obradovića 3, 21000 Novi Sad, Serbia |
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| Abstract: | A sensitive single-test-tube procedure for the fluorimetric determination of ng quantities of selenium with diaminonaphthalene, from small samples of animal origin is described. Several parameters related to the nitric/perchloric/sulphuric acid digestion, subsequent reduction and piazselenol formation are studied using blood as the matrix. The detection limit is 0.45 ng Se. The within-series precision for blood and heart tissue is 4.2% and 2.3% and between series is 5.0% and 3.6%, respectively. Recovery of added selenite and selenomethionine to blood, heart tissue and urine ranges from 98–101%. The correlation coefficient between the proposed and an electrothermal atomic absorption spectrometric method for serum samples is 0.997. This procedure is especially suitable for serial operation with a daily (8-h) throughput of 25 samples in duplicate. |
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