Evaluation of Antibacterial and Antifungal Properties of Low Molecular Weight Chitosan Extracted from Hermetia illucens Relative to Crab Chitosan |
| |
| Authors: | Adelya Khayrova Sergey Lopatin Balzhima Shagdarova Olga Sinitsyna Arkady Sinitsyn Valery Varlamov |
| |
| Institution: | 1.Institute of Bioengineering, Research Centre of Biotechnology, Russian Academy of Sciences, 119071 Moscow, Russia; (S.L.); (B.S.); (V.V.);2.Entoprotech Ltd., Skolkovo Innovation Centre, 121205 Moscow, Russia;3.Department of Chemistry, Moscow State University, 119991 Moscow, Russia; (O.S.); (A.S.) |
| |
| Abstract: | This study shows the research on the depolymerisation of insect and crab chitosans using novel enzymes. Enzyme preparations containing recombinant chitinase Chi 418 from Trichoderma harzianum, chitinase Chi 403, and chitosanase Chi 402 from Myceliophthora thermophila, all belonging to the family GH18 of glycosyl hydrolases, were used to depolymerise a biopolymer, resulting in a range of chitosans with average molecular weights (Mw) of 6–21 kDa. The depolymerised chitosans obtained from crustaceans and insects were studied, and their antibacterial and antifungal properties were evaluated. The results proved the significance of the chitosan’s origin, showing the potential of Hermetia illucens as a new source of low molecular weight chitosan with an improved biological activity. |
| |
| Keywords: | chitosan insect depolymerisation antibacterial activity antifungal activity Hermetia illucens |
|
|
