Anti-Phototoxicity Effect of Phenolic Compounds from Acetone Extract of Entada phaseoloides Leaves via Activation of COX-2 and iNOS in Human Epidermal Keratinocytes |
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| Authors: | Yanisa Mittraphab Yhiya Amen Maki Nagata Masako Matsumoto Dongmei Wang Kuniyoshi Shimizu |
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| Affiliation: | 1.Division of Systematic Forest and Forest Products Sciences, Department of Agro-Environmental Sciences, Graduate School of Bioenvironmental Sciences, Kyushu University, Fukuoka 819-0395, Japan; (Y.M.); (Y.A.); (M.N.); (M.M.); (D.W.);2.Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt |
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| Abstract: | The extract from Entada phaseoloides was employed as active ingredients of natural origin into cosmetic products, while the components analysis was barely reported. Using LC-DAD-MS/qTOF analysis, eleven compounds (1–11) were proposed or identified from acetone extract of E. phaseoloides leaves (AE). Among them, six phenolic compounds, protocatechuic acid (2), 4-hydroxybenzoic acid (3), luteolin-7-O-β-d-glucoside (5), cirsimaritin (6), dihydrokaempferol (9), and apigenin (10), were isolated by various chromatographic techniques. Protocatechuic acid (2), epicatechin (4), and kaempferol (11) at a concentration 100 μM increased the HaCaT cells viability of the UVB-irradiated cell without any cytotoxicity effect and reduced the expression of COX-2 and iNOS inflammation gene. Moreover, compounds 2 and 4 could have potent effects on cell migration during wound closure. These results suggest that compounds 2, 4, and 11 from AE have anti-photoaging properties and could be employed in pharmaceutical and cosmeceutical products. |
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| Keywords: | Entada phaseoloides photoprotection UVB HaCaT cells cosmeceuticals |
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