Preparative separation of atropine and scopolamine from Daturae metelis Flos using pH-zone-refining counter-current chromatography with counter-rotation and dual-mode elution procedure |
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Authors: | He Yutong Luo Jianguang Kong Lingyi |
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Institution: | Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing, PR China. |
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Abstract: | To isolate atropine and scopolamine from Daturae metelis Flos, three different elution modes have been applied in pH-zone-refining counter-current chromatography. These separations were performed with a two-phase solvent system composed of ethyl acetate/n-butanol/water (4:1:5 v/v) with 0.50% triethylamine in the organic phase and 0.15% hydrochloric acid in the aqueous phase. As a result, the best separation was obtained by counter-rotation and dual-mode elution procedure. In this new separation mode, the mobile phase and stationary phase were exchanged when the rotation direction was reversed. The two purified alkaloids (purity over 98% as determined by HPLC) were identified by ESI-MS, (1)H-NMR and (13)C-NMR. |
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Keywords: | Atropine Counter‐rotation Dual‐mode elution pH‐zone‐refining counter‐current chromatography Scopolamine |
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