| 磷钼蓝共振散射光谱分析法测定抗坏血酸 |
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| 引用本文： | 刘梯楼,孙双姣,袁利萍,张运良. 磷钼蓝共振散射光谱分析法测定抗坏血酸[J]. 光谱实验室, 2010, 27(5): 2060-2063 |
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| 作者姓名： | 刘梯楼 孙双姣 袁利萍 张运良 |
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| 作者单位： | 1. 邵阳医学高等专科学校,湖南省邵阳市宝庆西路18号,422000
2. 中南林业科技大学理学院,长沙市韶山南路498号,410004 |
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| 基金项目： | 邵阳市科技局科技项目，湖南省教育厅优秀青年项目 |
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| 摘 要： | 在硫酸介质中,抗坏血酸可以定量地将磷钼酸铵还原成磷钼蓝,磷钼蓝在390nm波长处产生强烈的共振光散射峰,在0.15—5.00mg/L范围内,随着抗坏血酸浓度的增加,共振散射强度线性增强,回归方程为ΔIRS=21.45ρ+2.63,相关系数为0.9986,检出限0.12mg/L。由此建立了一种简单、灵敏检测抗坏血酸的方法,该方法用于测定饮料中的抗坏血酸,具有方便、快速的特点和较高的准确度和重现性。
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| 关 键 词： | 抗坏血酸 磷钼蓝 共振散射光谱法 |
Determination of Ascorbic Acid by Resonance Scattering Spectrometry with Phosphomolybdate Blue |
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| LIU Ti-Lou,SUN Shuang-Jiao,YUAN Li-Ping,ZHANG Yun-Liang. Determination of Ascorbic Acid by Resonance Scattering Spectrometry with Phosphomolybdate Blue[J]. Chinese Journal of Spectroscopy Laboratory, 2010, 27(5): 2060-2063 |
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| Authors: | LIU Ti-Lou SUN Shuang-Jiao YUAN Li-Ping ZHANG Yun-Liang |
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| Affiliation: | LIU Ti-Lou SUN Shuang-Jiao YUAN Li-Pinga ZHANG Yun-Liang(Shaoyang Medical College,Shaoyang,Hunan 422000,P.R.China)a(College of Science,Central South University of Forestry and Technology,Changsha 410004,P.R.China) |
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| Abstract: | In the medium of H2SO4,ascorbic acid can reduce phosphomolybdate ammonium quantitatively to phosphomolybdate blue,which exhibits strong resonance scattering peak at 390nm.The intensity of resonance scattering is proportional to the concentration of ascorbic acid in the range of 0.15—5.00mg/L,with regression equation of ΔIRS=21.45ρ＋2.63,relative coefficient of 0.9986,detection limit of 0.12mg/L.The method was applied successfully for the analysis of ascorbic acid in drink,easily and conveniently.The accuracy and the repeatability are satisfactory. |
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| Keywords: | Ascorbic Acid Phosphomolybdate Blue Resonance Scattering Spectrometry |
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