| 一种考马斯亮蓝G-250快速染色法在SDS-PAGE中的应用 |
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| 引用本文： | 吴远双,宋玉竹.一种考马斯亮蓝G-250快速染色法在SDS-PAGE中的应用[J].光谱实验室,2013(6):3109-3113. |
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| 作者姓名： | 吴远双 宋玉竹 |
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| 作者单位： | 昆明理工大学生命科学与技术学院,昆明市呈贡区景明南路727号650500 |
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| 基金项目： | 国家自然科学基金（31000960） |
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| 摘 要： | 对SDS聚丙烯酰胺凝胶电泳中一种仅加入3mmol/L盐酸的考马斯亮蓝G-250染色液配合微波加热的快速染色方法的具体条件进行了研究.该方法能在1h内完成,比常规考马斯亮蓝R-250染色法短得多.同时未加入有机溶剂和其他酸,对人体几乎无害,对环境污染小.而其灵敏度与常规考马斯亮蓝R-250染色法相近,适于在实验教学和科研方面进行推广应用.
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| 关 键 词： | 十二烷基硫酸钠聚丙烯酰胺凝胶电泳 考马斯亮蓝G-250 快速染色 |
Application of a Fast Protein Staining Method with CBBG-250 in SDS-PAGE |
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| WU Yuan-Shuang,SONG Yu-Zhu.Application of a Fast Protein Staining Method with CBBG-250 in SDS-PAGE[J].Chinese Journal of Spectroscopy Laboratory,2013(6):3109-3113. |
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| Authors: | WU Yuan-Shuang SONG Yu-Zhu |
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| Institution: | (Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650500, P. R. China) |
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| Abstract: | The dyeing conditions for a heating with Coomassie Brilliant Blue G-250 fast protein staining method, combining microwave-（CBB G-250）, were investigated in SDS-PAGE. The whole procedure, which can be finished within 1 hour, is much faster than the conventional CBB R-250 staining protocol. No organic solvent and other acids but 3mmol/L hydrochloric acid was used in the method, which is henefical to operators and environment-friendly. Moreover, the obtained sensitivity was similar with that of the classic staining protocol. The method is applicable to the rapid analysis of protein electrophoresis in the experimental teaching and scientific research. |
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| Keywords: | SDS Polyacrylamide Gel Electrophoresis Coomassie Brilliant Blue G-250 FastProtein Staining |
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