| HPLC法测定大鼠盲肠内容物中5-氨基水杨酸的含量 |
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| 引用本文： | 田睿,刘静,郭锋,谷萌辉,张良珂.HPLC法测定大鼠盲肠内容物中5-氨基水杨酸的含量[J].光谱实验室,2013(6):3036-3039. |
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| 作者姓名： | 田睿 刘静 郭锋 谷萌辉 张良珂 |
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| 作者单位： | [1]重庆医科大学实验教学中心,重庆市渝中区医学院路1号400016 [2]重庆医科大学药学院,重庆市渝中区医学院路1号400016 |
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| 基金项目： | 重庆市自然科学基金（CSTC2012jjA10021）;高等学校博士学科点专项科研基金（20125503120003）;重庆医科大学大学生科研与创新实验项目（201244,201229,201217）;海扶之星学生科研基金项目（XS201309）;重庆市教育委员会科学技术研究项目（KJ110323） |
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| 摘 要： | 建立高效液相色谱法测定5-氨基水杨酸在大鼠盲肠内容物中的含量.色谱柱为依利特C18柱（Hypersil BDS,200mm×4.6mm,5μm）,流动相为磷酸盐缓冲液（含0.01mol·L^-1四丁基溴化铵,pH 6.6）∶甲醇=75∶25 （V/V）,流速1.0mL·min-1,检测波长240nm.实验结果表明大鼠盲肠内容物中的内源性物质不干扰测定;线性范围为1.0-64.0μg·mL^-1（r=0.9994）；样品日内日间精密度的RSD均小于2％,回收率在99％-101％之间.本法快捷、灵敏、准确,可用于5-氨基水杨酸大鼠盲肠内容物药物浓度的测定.
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| 关 键 词： | 5-氨基水杨酸 高效液相色谱法 大鼠盲肠内容物 |
Determination of 5-Aminosalicylic Acid in Rat Cecal Content Solution by HPLC |
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| The Experimental Teaching Centre. Chongqhlg Medical University,Chongqing,P. R. China ;School of pharmacy. Chongqing Medical University,Chongqing,Chongqing,P. R. China TIAN Rui,LIU Jing,GUO Feng,GU Meng-Hui,ZHANG Liang-Ke.Determination of 5-Aminosalicylic Acid in Rat Cecal Content Solution by HPLC[J].Chinese Journal of Spectroscopy Laboratory,2013(6):3036-3039. |
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| Authors: | The Experimental Teaching Centre Chongqhlg Medical University Chongqing P R China ;School of pharmacy Chongqing Medical University Chongqing Chongqing P R China TIAN Rui LIU Jing GUO Feng GU Meng-Hui ZHANG Liang-Ke |
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| Institution: | ( The Experimental Teaching Centre. Chongqhlg Medical University, Chongqing 400016, P. R. China ;School of pharmacy. Chongqing Medical University, Chongqing , Chongqing 400016, P. R. China )TIAN Rui, LIU Jing ,GUO Feng,GU Meng-Hui, ZHANG Liang-Ke |
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| Abstract: | The aim of this paper is to establish a HPLC method for the determination of 5- aminosaticylic acid in rat cecal content solution. The study was performed on Elite C18 column （Hypersil BDS, 200mm × 4. 6mm, 5μm） with the mobile phase of PBS buffer （containing 0. 01mol- L^-1 tetrabutyl ammonium bromide,pH6.6）-methanol （75 ： 25,V/V） at a flow rate of 1.0mL · min-1. The eluent＇was detected at 240nm. The results of chromatographic method showed a good specificity between analytes and matrix. It showed good linerity over a range of 1. 0-64. 0μg · mL^-1 （r = 0. 9994）. The method recovery was between 99% and 101%,and inter-day and intra-day precision RSDs were all less than 2. 0M. The developed method was fast, sensitive and accurate for determination of 5-aminosalicylic acid in rat cecal content solution. |
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| Keywords: | 5-Aminosalicylic Acid HPLC Rat Cecal Content Solution |
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