| Mn（Ⅱ）与BSA相互作用的热力学特征 |
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| 引用本文： | 荣先国,高宗华,黄玉玲,张怀斌.Mn（Ⅱ）与BSA相互作用的热力学特征[J].光谱实验室,2013(6):3130-3133. |
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| 作者姓名： | 荣先国 高宗华 黄玉玲 张怀斌 |
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| 作者单位： | 滨州医学院（烟台校区）药学院,山东省烟台市观海路346号264003 |
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| 基金项目： | 山东省高等学校科技计划资助项目（J12LD55）;滨州医学院科研专项资助项目（BY2009KJ30） |
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| 摘 要： | 运用荧光光谱法研究了锰（Ⅱ,Mn2＋）与牛血清白蛋白（Bovine Serum Albumin,BSA）的相互作用.随着Mn2＋浓度的增大,BSA的荧光强度降低,最大发射峰蓝移2nm,根据Stern-Volmer方程求出了Mn2＋与BSA作用的猝灭常数,其猝灭机制为静态猝灭.根据热力学方程求得了290K和308K时的热力学参数△H、△G和△S,结果表明温度升高Mn2＋与BSA的作用增强,二者之间的作用力主要为疏水作用和静电作用.
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| 关 键 词： | Mn2＋ 牛血清白蛋白 荧光猝灭 热力学特征 |
Thermochemical Characteristics on the Reaction of Manganese Ion with Bovine Serum Albumin |
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| RONG Xian-Guo,GAO Zong-Hua,HUANG Yu-Ling,ZHANG Huai-Bin.Thermochemical Characteristics on the Reaction of Manganese Ion with Bovine Serum Albumin[J].Chinese Journal of Spectroscopy Laboratory,2013(6):3130-3133. |
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| Authors: | RONG Xian-Guo GAO Zong-Hua HUANG Yu-Ling ZHANG Huai-Bin |
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| Institution: | ( Pharmaceutical College, Binzhou Medical University, Yantai Shandong 264003, P. R. China ) |
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| Abstract: | The interaction mechanism of manganese ion （ Ⅱ ,Mn^2＋） and bovine serum albumin （BSA） was studied by the fluorescence spectroscopy. The fluorescence spectroscopy of BSA quenched with adding the concentration of Mn^2＋. The maximum emission wavelength of BSA was blueshift 2nm. On the basis of Stern-Volmer equation,the quenching constant was obtained,and the quenching mechanism of BSA was a static quenching machenism. Judging by relevant thermodynamics formula, the thermodynamic parameters （△H, AG and △S） were calculated. The results suggested that the conjugation＇ of Mn^2＋ and BSA enhanced with elevating the tempreture and the acting force was mainly hydrophobic force and electrostatic interaction. |
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| Keywords: | Manganese Ion Bovine Serum Albumin Fluorescence Quenching ThermodynamicCharacteristics |
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