检测维生素C的囊泡荧光传感器的制备

利用合成的含有识别基团苯硼酸和荧光读出基团萘的新型双亲化合物(DNMPBA)在THF/水选择性溶剂中自组织成囊泡,囊泡的相变温度为56.8℃;当向囊泡体系加维生素C时,DNMPBA囊泡中的萘生色基在345nm的荧光峰强度急剧减弱.荧光强度减弱归于所形成的硼酸酯增强了DNMPBA双亲化合物中一个氧原子孤对电子对萘生色基的淬灭作用.DNMPBA囊泡与维生素C的相互作用而导致体系荧光强度变化,使该体系有可能应用于检测生物物质如维生素C的化学传感器.

Preparation of Fluorescent Vesicular Sensor for Determination of Vitamin C

A novel amphiphile containing two functional groups of both naphthalene and phenylboronic acid,p-（（5-dodecyloxy-1-oxy） naphthalene） methyl-phenylboronic acid （DNMPBA） was synthesized.The amphiphile has self-organized into vesicles in the selective solution of THF/water （φ=0.6） buffered at pH 6.86.The Tc of the vesicles from crystal to liquid crystal is 56.8 ℃.Addition of vitamin C to the vesicular solution,the fluorescent intensities of naphthalene in DNMPBA vesicles centred at 345 nm weakened dramatically with increasing concentration of vitamin C.The decreace of fluorescence intensity is ascribed to boronic ester formation which might largen the photoinduced electron transfer （PET） progress.The vesicular sensor through the interactions between boronic acid and vitamin C displayed at a biomimetic membrane interface can be detected by means of fluorescence intensities.The results suggest that the fluorescent vesicles might be used as biosensors to determine vitamin C.