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A simple and rapid chemical method for the determination of cephalosporins |
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| Authors: | Claus Lubbe Yong-Qiang Shen Arnold L. Demain |
| Affiliation: | 1. Fermentation Process Development, Pharmaceutical Research Division, Schering-Plough Union, NJ 2. Department of Microbiology, Institute of Plant Physiology, Chinese Academy of Sciences, Shanghai, The People’s Republic of China 3. Fermentation Microbiology Laboratory, Department of Applied Biological Sciences, Massachusetts Institute of Technology, Cambridge, MA |
| Abstract: | A simple and rapid chemical assay for cephalosporins is described. It is a simple modification of the colorimetric determination of penicillins in which the narrow spectrum beta-lactamase (penicillinase) is replaced by a broad spectrum beta-lactamase (cephalosporinase) produced by Enterobacter cloacae. The method can be used for assay of fermentation broths as well as for pure cephalosporins. |
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