| 催化反应热诱导激光光偏转分析法测定尿素 |
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| 引用本文： | 阎宏涛 朱小云. 催化反应热诱导激光光偏转分析法测定尿素[J]. 分析化学, 1997, 25(7): 804-806 |
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| 作者姓名： | 阎宏涛 朱小云 |
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| 作者单位： | 西北大学化学系!西安710069 |
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| 基金项目： | 国家教委优秀年轻教师基金资助课题 |
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| 摘 要： | 报道用催化反应热诱导激光光偏转法测定痕量尿素。发现将脲酶制成一定浓度的乙醇浓液能显著地增强激光光偏转信号强度。探讨了增强作用机理以及光偏转号强度与尿素浓度的关系，并应用于尿样中尿素含量的测定，结果满意。
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| 关 键 词： | 酶催化 激光光偏转 尿素 反应热诱导 |
Determination of Urea Based on Reaction Heat-Induced Laser Beam Deflection |
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| Yan Hongtao,Zhu Xiaoyun. Determination of Urea Based on Reaction Heat-Induced Laser Beam Deflection[J]. Chinese Journal of Analytical Chemistry, 1997, 25(7): 804-806 |
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| Authors: | Yan Hongtao Zhu Xiaoyun |
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| Abstract: | A novel analytical method for determination of urea based on reaction heat-in- duced laser beam deflection is presented. The urease catalyzes the hydrolysis of urea. It was found that the signal could be enhanced if an ethanol solution of urease was used for the reac- tion. The enhancement mechanism of ethanol for the beam deflection signal and the relation between the beam deflection signal and the concentration of urea were investigated. With the present method, the urea in urine was determined with satisfactory results. |
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| Keywords: | Enzyme catalysis reaction laser beam deflection urea |
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