| 水-乙醇混合溶剂中过氧化氢酶活性与构象的研究 |
| |
| 引用本文： | 易平贵,俞庆森,胡新根,商志才,梅明辉,林瑞森. 水-乙醇混合溶剂中过氧化氢酶活性与构象的研究[J]. 化学学报, 2000, 58(6): 652-655 |
| |
| 作者姓名： | 易平贵 俞庆森 胡新根 商志才 梅明辉 林瑞森 |
| |
| 作者单位： | 浙江大学化学系.杭州(310027) |
| |
| 基金项目： | 国家自然科学基金，29633020， |
| |
| 摘 要： | 用微量热法和荧光法分别测定了水-乙醇混合溶剂中过氧化氢酶活性与构象的变化。结果表明，随着乙醇浓度的增大，过氧化氢酶酶促反应的米氏常数K~m有所增大，而反应速率常数k~2及酶的催化活性则明显降低；336nm处的相对荧光强度不断增强，酶分子的构象发生了变化，其结构渐趋松散。乙醇对过氧化氢酶活性的影响乃是乙醇的竞争性抑制和溶剂效应引起的酶构象变化共同作用的结果。
|
| 关 键 词： | 水 乙醇 过氧化氢酶 构象 催化活性 |
| 修稿时间： | 1999-09-22 |
Activity and conformation of catalase in water-ethanol mixtures |
| |
| YI Ping-Gui,YU Qing-Sen,Hu Xin-Gen,SHANG Zhi-Cai,MEI Ming-hui,LIN Rui-Sen. Activity and conformation of catalase in water-ethanol mixtures[J]. Acta Chimica Sinica, 2000, 58(6): 652-655 |
| |
| Authors: | YI Ping-Gui YU Qing-Sen Hu Xin-Gen SHANG Zhi-Cai MEI Ming-hui LIN Rui-Sen |
| |
| Affiliation: | Zhejiang Univ, Dept Chem.Hangzhou(310027) |
| |
| Abstract: | The activity and conformation changes of catalase in water - ethanol mixtures were measured at 298.15K by means of microcalorimetry and fluorescence spectroscopy. The results showed that the Michaelis constant Km of catalase catalyzed reaction as well as the relative fluorescence intensity of catalase increased the rate constant k2 and the activity of catalase decreased with increasing the ethanol concentration. The conformation of catalase was changed with losing activity at the various concentration of ethanol. The lose of activity of catalase in water - ethanol mixtures was caused by the inhibition of ethanol and the conformational change of catalase. |
| |
| Keywords: | catalase microcalorimetry fluorescence spectrum water - ethanol mixtures conformation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《化学学报》浏览原始摘要信息 |
|
点击此处可从《化学学报》下载全文 |
