PURIFICATION OF L-METHIONINE AND N-ACETYL-D-METHIONINE FROM THE MIXTURE OF ENZYMATICALLY DEACYLATED N-ACETYL-DL-METHIONINE |
| |
Authors: | Yan Xiaomin ZHAO Lin SHAO Jianhui TAN Xin Song Zhengxiao |
| |
Affiliation: | 1. School of Chemical Engineering and Technology, Tianjin University Tianjin 300072, China 2. Slichem Liquid Material Co. Ltd., Shijiazhuang, 050061, China |
| |
Abstract: | N-acetyl-D-methionine, NaAc and the remains of N-acetyl-L-methionine dramatically affect the purification of L-methionine when purified from the mixture of enzymatically deacylated N-acetyl-DL-methionine, leading to a low yield conventionally. Here, this paper reports a successful separation and purification of both L-methionine and N-acetyl-D-methionine by an H ion-exchange column. The pH, L-Met concentration and the ratio between the content of sodium cation and the ion-exchange capacity were optimized to obtain the maximum yield. Experimental results indicate that, under the optimized conditions, the yields of L-methionine and N-acetyl-D-methionine can reach as high as 85% and 75%, respectively. |
| |
Keywords: | Enzymatic deacylation hydrolysed of N-acetyl-DL-methionine Strong acid ion-exchanger separation of L-methionine and N-acetyl-D-methionine |
本文献已被 维普 万方数据 等数据库收录! |
|