Enzymes immobilized on montmorillonite: comparison of performance in batch and packed-bed reactors |
| |
Authors: | Sanjay Gopinath and Sankaran Sugunan |
| |
Institution: | (1) Department of Applied Chemistry, Cochin University of Science and Technology, Kochi-682022, India;(2) Department of Applied Chemistry, Cochin University of Science and Technology, Kochi-682022, India |
| |
Abstract: | Summary The enzymes a-amylase, invertase and glucoamylase were immobilized on acid activated montmorillonite using two techniques,
viz. adsorption and covalent binding, and their activities were tested in a batch and packed-bed reactor and were compared. The
packed-bed reactor showed an improved performance for all immobilized enzymes, which was attributed to lowering of diffusional
restrictions to mass transfer. Lower activity in case of batch reactor for immobilized invertase was due to a combined effect
of loss of native conformation of enzyme on account of immobilization and mass transfer resistances due to improper diffusion
of substrate to the active site of enzyme. For immobilized glucoamylase, the packed-bed reactor demonstrated exceptionally
high activity that was very close to the free enzyme. Covalently bound glucoamylase showed higher activity than the free enzyme. |
| |
Keywords: | adsorption packed-bed reactor batch reactor covalent binding Immobilization |
本文献已被 SpringerLink 等数据库收录! |
|