| 应用二阶校正算法-荧光分光光度法同时测定血浆中诺氟沙星和左氧氟沙星 |
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| 引用本文： | 胡乐乾,尹春玲,魏志垒.应用二阶校正算法-荧光分光光度法同时测定血浆中诺氟沙星和左氧氟沙星[J].理化检验(化学分册),2009(12). |
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| 作者姓名： | 胡乐乾 尹春玲 魏志垒 |
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| 作者单位： | 河南工业大学化学化工学院; |
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| 基金项目： | 河南教育厅科研基金资助(2008A150009); 河南工业大学博士基金(2006BS010); 湖南大学国化学、生物传感与国家重点实验室开放基金(2007009) |
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| 摘 要： | 应用三维荧光技术,结合化学计量学中的二阶校正算法,在诺氟沙星和左氧氟沙星荧光光谱严重重叠,以及干扰物质存在下对血浆中两种喹诺酮类药物进行了同时定量分析。三维荧光激发波长范围265～510nm,发射波长范围300～650 nm。交替不对称三线性分解(AATLD)算法解析得到的诺氟沙星和左氧氟沙星的平均回收率分别为96.9%和103.9%。方法前处理简单、不需预先分离、可以快速定量分析血浆中光谱相互干扰的待测药物的含量。
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| 关 键 词： | 荧光光度法 二阶校正 诺氟沙星 左氧氟沙星 |
Simultaneous Fluorospectrophotometric Determination of Norfloxacin and Levofloxacin in Plasma Using the Algorithm of 2nd Order Calibration |
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| HU Le-qian,YIN Chun-Ling,WEI Zhi-leiCollege of Chem. , Chem. Eng.,Henan University of Industry,Zhengzhou ,China.Simultaneous Fluorospectrophotometric Determination of Norfloxacin and Levofloxacin in Plasma Using the Algorithm of 2nd Order Calibration[J].Physical Testing and Chemical Analysis Part B:Chemical Analgsis,2009(12). |
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| Authors: | HU Le-qian YIN Chun-Ling WEI Zhi-leiCollege of Chem Chem Eng Henan University of Industry Zhengzhou China |
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| Institution: | HU Le-qian,YIN Chun-Ling,WEI Zhi-leiCollege of Chem. and Chem. Eng.,Henan University of Industry,Zhengzhou 450001,China |
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| Abstract: | Simultaneous determination of norfloxacin and levofloxacin by fluorospectrophotometry with scanning in the excitation wavelength range of 265-510 ran and in the emission wavelength range of 300-650 run. Data of fluorescence intensity measured were treated by the algorithms of 2nd order calibration and 3-dimensional data array analysis. As analyzed by the algorithm of AATLD, values of average recovery of 96. 9% (for norfloxacin) and 103. 9% (for levofloxacin) were found. It was shown by the analytical result... |
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| Keywords: | Fluorospectrophotometry 2nd Order calibration Norfloxacin Levofloxacin |
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