| 膜质毛细管浓缩酶联光度法在线测定水样中总酚 |
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| 引用本文： | 金谷,姚奇志,杨键,张蕾.膜质毛细管浓缩酶联光度法在线测定水样中总酚[J].理化检验(化学分册),2009(8). |
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| 作者姓名： | 金谷 姚奇志 杨键 张蕾 |
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| 作者单位： | 中国科学技术大学化学系; |
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| 摘 要： | 提出用已二酰二氯作交联剂,聚乙烯醇(PVA)涂层改性聚四氟乙烯(PTFE)膜质毛细管,并用于多酚氧化酶(PPO)的固定,在线分析过程采用流动注射系统。这种经修饰的PTFE膜质毛细管可催化水溶液中酚的氧化,氧化产物与染料4-氨基安替比林偶合生成的有色化合物再经未涂层的PTFE膜质毛细管循环15 min分离富集,用乙醇洗脱后进入流通池在510 nm波长处进行在线检测。对在线酶催化反应和显色反应的时间,试验进入反应圈1及2时的流速,淋洗时,淋洗液的流速等均给了最佳参数。反应系在pH 6.8的磷酸盐缓冲介质中进行。该方法的线性范围为0.5～60μg·L~(-1),水样中常见的离子和化合物不干扰酚的测定。应用此法分析了地下水及自来水样中痕量酚,所得结果与国标标准方法所得结果之间的相对误差在1.8%～3.3%范围内。
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| 关 键 词： | 流动注射在线光度法 酶催化氧化 多酚氧化酶 PVA-PPO涂层 PTFE膜质毛细管 酚 水样 |
FI-Online Enzymatic Oxidation and Preconcentration by PVA Modified Membranous Capillary-Photometric Determination of Total Phenols in Water |
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| JIN Gu,YAO Qi-zhi,YANG Jian,ZHANG Lei.FI-Online Enzymatic Oxidation and Preconcentration by PVA Modified Membranous Capillary-Photometric Determination of Total Phenols in Water[J].Physical Testing and Chemical Analysis Part B:Chemical Analgsis,2009(8). |
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| Authors: | JIN Gu YAO Qi-zhi YANG Jian ZHANG Lei |
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| Institution: | JIN Gu,YAO Qi-zhi,YANG Jian,ZHANG Lei (Dept.of Chemistry,University of Science and Technology of China,Hefei 230026,China) |
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| Abstract: | Polyphenol oxidase(PPO) was immobilized on the surface of PTFE membranous capillary by using adipoyldichloride as crosslinking agent.A FI-manifold was used for the on-line analytical process.Phenols in water sample were enzymatically oxidized when cycling in the PVA-PPO modified PTFE membranous capillary,and the oxidation products of phenols were reacted with 4-aminoantipyrine to give a colored compound.The colored solution of definite volume was enriched by cycling for 15 min in the un-modified PTFE membra... |
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| Keywords: | FI-online photometry Enzymatic oxidation Polyphenol oxidase PVA-PPO coating Modified PTFE membranous capillary Phenols Water |
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