DNA cleavage promoted by Cu2+ complex of cyclen containing pyridine subunit |
| |
| Authors: | Ying Li Xiao-Min Lu Xin Sheng Guo-Yuan Lu Ying Shao Qiang Xu |
| |
| Institution: | (1) Department of Chemistry, State Key Laboratory of Coordination Chemistry, Nanjing University, Nanjing, 210093, P.R. China;(2) State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210093, P.R. China |
| |
| Abstract: | The tetraazamacrocycle crown ether (cyclen) containing two pyridine subunits was prepared by a modified procedure and the
interaction of its metal complexes with DNA was studied by agarose gel electrophoresis analysis. The results indicate that
the Cu2+ complex as nuclease model can promote the hydrolysis of phosphodiester bond of supercoiled DNA. The rate of degradation of
the supercoiled DNA (form I) to nicked DNA (form II) obtained at physiological condition in the presence of 2.14 mM Cu2+ complex is 2.31 × 10–3 min−1. The dependence of the rate of supercoiled DNA cleavage from the complex concentration shows an unusual profile and a hydrolytic
cleaving mechanism of two monometallic complexes through cooperation from two-point binding to DNA is proposed.
Graphical abstract
DNA cleavage promoted by metal complex of cyclen containing pyridine subunit
Ying Li, Xiao-Min Lu, Xin Sheng, Guo-Yuan Lu*, Ying Shao and Qiang Xu*
The copper complex of tetraazamacrocycle crown ether (cyclen) containing two pyridine subunits can promote the hydrolysis
of phosphodiester bond of supercoiled DNA and a hydrolytic mechanism of two
monometallic complexes through cooperation from two-point binding to DNA is proposed. 
|
| |
| Keywords: | Cyclen DNA cleavage Phosphodiester Enzyme mimic Electrophoresis |
| 本文献已被 SpringerLink 等数据库收录！ |
|
