| 小檗碱与牛血清白蛋白相互作用的核磁共振研究 |
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| 引用本文： | 吴丽敏,张美玲,娄依依,李旸,赵良才,黄可新,高红昌.小檗碱与牛血清白蛋白相互作用的核磁共振研究[J].分析化学,2011,39(8):1223-1227. |
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| 作者姓名： | 吴丽敏 张美玲 娄依依 李旸 赵良才 黄可新 高红昌 |
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| 作者单位： | 1. 温州医学院药学院,温州,325035
2. 中科院上海药物研究所新药研究国家重点实验室,上海,201203 |
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| 基金项目： | 国家自然科学基金(No.20705037); 浙江省医药卫生专项(No.2010QNA016)资助 |
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| 摘 要： | 采用核磁共振波谱技术(NMR)研究小檗碱与牛血清白蛋白(BSA)的相互结合作用.比较不同浓度小檗碱在一定浓度的BSA溶液中的化学位移、弛豫时间和扩散系数的变化情况.研究表明,小檗碱环上芳香质子与BSA的结合作用较强,其它烷基质子与BSA结合作用较弱;BSA的结合使得小檗碱各个质子的1HT1弛豫时间明显减少,质子在溶液中...
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| 关 键 词： | 核磁共振 小檗碱 牛血清白蛋白 弛豫时间 自扩散系数 |
Nuclear Magnetic Resonance Studies on Interactions of Berberine and Bovine Serum Albumin |
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| WU Li-Min,ZHANG Mei-Ling,LOU Yi-Yi,LI Yang,ZHAO Liang-Cai,HUANG Ke-Xin,GAO Hong-Chang.Nuclear Magnetic Resonance Studies on Interactions of Berberine and Bovine Serum Albumin[J].Chinese Journal of Analytical Chemistry,2011,39(8):1223-1227. |
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| Authors: | WU Li-Min ZHANG Mei-Ling LOU Yi-Yi LI Yang ZHAO Liang-Cai HUANG Ke-Xin GAO Hong-Chang |
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| Institution: | WU Li-Min1,ZHANG Mei-Ling1,LOU Yi-Yi1,LI Yang1,ZHAO Liang-Cai2,HUANG Ke-Xin*1,GAO Hong-Chang*1 1(School of Pharmacy,Wenzhou Medical College,Wenzhou 325035) 2(Shanghai Institute of Materia Medica,Shanghai 201203) |
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| Abstract: | 1H nuclear magnetic resonance chemical shift,relaxation time and diffusion coefficient measurements were carried out to study the interaction on the affinity binding of Berberine,an antibacterial drug,to bovine serum albumin(BSA).The results showed that the protons of aromatic ring of Berberine were more favorable to form Berberine-BSA complex than those of aliphatic groups.BSA combination resulted in significantly decrease in the relaxation time of Berberine protons,which suggests that the motion of these ... |
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| Keywords: | Nuclear magnetic resonance(NMR) Berberine Bovine serum albumin Relaxation time Self-diffusion coefficient |
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