| 大肠杆菌代谢物组成的核磁共振分析 |
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| 引用本文： | 叶央芳,张利民,安艳捧,豪富华,唐惠儒. 大肠杆菌代谢物组成的核磁共振分析[J]. 分析化学, 2011, 39(8): 1186-1194. DOI: 10.3724/SP.J.1096.2011.01186 |
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| 作者姓名： | 叶央芳 张利民 安艳捧 豪富华 唐惠儒 |
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| 作者单位： | 1. 波谱与原子分子物理国家重点实验室,武汉磁共振中心,中国科学院武汉物理与数学研究所,武汉,430071;宁波大学应用海洋生物技术教育部重点实验室,宁波,315211
2. 波谱与原子分子物理国家重点实验室,武汉磁共振中心,中国科学院武汉物理与数学研究所,武汉,430071 |
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| 基金项目： | 国家杰出青年科学基金(No.20825520); 传染病专项基金(No.2009ZX10601); 浙江省自然科学基金(No.Y3090046); 宁波大学学科项目(No.xkl09120)资助项目 |
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| 摘 要： | 为了认识重要模式生物大肠杆菌的内源性代谢物组成,本研究综合使用一维和二维高分辨核磁共振波谱技术,系统测定了大肠杆菌的代谢物种类和含量.结果表明:核磁共振方法可检测到分别来自多个代谢途径的氨基酸、有机羧酸、糖类、核苷及其衍生物等40多种高丰度代谢物.葡萄糖是稳定期大肠杆菌中含量最丰富的物质;谷氨酸、甜菜碱、腐胺和核糖-5...
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| 关 键 词： | 大肠杆菌 代谢组 核磁共振(NMR) |
Nuclear Magnetic Resonance for Analysis of Metabolite Composition of Escherichia coli |
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| YE Yang-Fang,ZHANG Li-Min,AN Yan-Peng,HAO Fu-Hua,TANG Hui-Ru. Nuclear Magnetic Resonance for Analysis of Metabolite Composition of Escherichia coli[J]. Chinese Journal of Analytical Chemistry, 2011, 39(8): 1186-1194. DOI: 10.3724/SP.J.1096.2011.01186 |
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| Authors: | YE Yang-Fang ZHANG Li-Min AN Yan-Peng HAO Fu-Hua TANG Hui-Ru |
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| Affiliation: | YE Yang-Fang1,2,ZHANG Li-Min1,AN Yan-Peng1,HAO Fu-Hua1,TANG Hui-Ru*1 1(State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics,Wuhan Institute of Physics and Mathematics,Chinese Academy of Sciences,Wuhan 430071) 2(Key Laboratory of Applied Marine Biotechnology,Ministry of Education,Ningbo University,Ningbo 315211) |
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| Abstract: | To understand the composition of endogenous metabolites of Escherichia coli,an important model organism,a series of high-resolution NMR spectroscopic methods were employed to analyze the metabolites of this strain qualitatively and quantitatively.We found that E.coli metabolome was dominated by more than 40 abundant metabolites including amino acids,organic acids,sugars,nucleoside and its derivatives involving many different metabolic pathways.We further found that glucose level was the highest in stationar... |
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| Keywords: | Escherichia coli Metabolome Nuclear magnetic resonance |
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