| (3+1)机制形成端粒四联体并环结构的电化学研究 |
| |
| 引用本文： | 杨茜璐,周楠迪,张冬梅. (3+1)机制形成端粒四联体并环结构的电化学研究[J]. 分析科学学报, 2011, 27(3): 289-292 |
| |
| 作者姓名： | 杨茜璐 周楠迪 张冬梅 |
| |
| 作者单位： | 南京大学生物化学系医药生物技术国家重点实验室;江南大学生物工程学院工业生物技术教育部重点实验室; |
| |
| 基金项目： | 国家自然科学基金(No.20805020); 教育部科学技术研究重点项目(No.109079); 江苏省自然科学基金(No.BK2008268); 江南大学校级青年基金(2007LQN024) |
| |
| 摘 要： | 本文用电化学方法对(3+1)机制形成的"四联体并环结构"的现象进行了研究.通过紫外可见光谱法,计时库仑法和差分脉冲单扫描法对形成条件进行了优化,通过循环伏安法验证了四联体结构的形成,并得到了它的直接电化学信号.实验发现该结构对微量过氧化氢不稳定,进一步通过光谱实验证明并分析了原因.本文对该机制参与端粒环形成的假说进行了...
|
| 关 键 词： | 电化学 端粒 端粒环 四联体 血红素 |
Electrochemical Study of a Telomeric Quadruplex-and-loop Structure Formed by(3+1) Mechanism |
| |
| YANG Qian-lu,ZHOU Nan-di,ZHANG Dong-mei. Electrochemical Study of a Telomeric Quadruplex-and-loop Structure Formed by(3+1) Mechanism[J]. Journal of Analytical Science, 2011, 27(3): 289-292 |
| |
| Authors: | YANG Qian-lu ZHOU Nan-di ZHANG Dong-mei |
| |
| Affiliation: | YANG Qian-lu1,ZHOU Nan-di2,ZHANG Dong-mei*1(1.Department of Biochemistry and Natinal Key Laboratory of Pharmaceutical Biotechnology,Nanjing University,Nanjing 210093,2.School of Biotechnology and the Key Laboratory of Industrial Biotechnology,Ministry of Education,Jiangnan University,Wuxi 214122) |
| |
| Abstract: | Electrochemical techniques were used to investigate the formation of quadruplex-and-loop structure via a(3+1) mechanism in this paper.Spectroscopic methods,chronocoulometric methods and differential pulse voltammetry methods were firstly used to find optimal conditions including the reactive buffer,time and DNA-modification density.Next,cyclic voltammetry studies were performed to prove the formation of the quadruplex-and-loop structure,and the direct electrochemical signal was obtained but found that it wa... |
| |
| Keywords: | Electrochemistry Telomere T-loop Quadruplex Hemin |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
