| 糖尿病大鼠肝细胞膜中胰岛素受体巯基的检测 |
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| 引用本文： | 葛伊莉,盛勤芳,宋功武.糖尿病大鼠肝细胞膜中胰岛素受体巯基的检测[J].分析科学学报,2011,27(2):227-230. |
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| 作者姓名： | 葛伊莉 盛勤芳 宋功武 |
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| 作者单位： | 湖北大学化学化工学院;湖北轻工职业技术学院; |
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| 摘 要： | 采用四氧嘧啶腹腔注射法建立糖尿病大鼠实验模型,用巯基试剂盒紫外可见分光光度法研究四氧嘧啶所致糖尿病模型大鼠肝脏细胞膜中胰岛素受体的性质变化.实验结果表明,糖尿病大鼠肝脏组织细胞膜胰岛素受体的自由巯基含量明显低于正常大鼠,每克蛋白质降低了22%(P＜0.05),提示糖尿病大鼠肝细胞膜胰岛素受体存在氧化损伤.
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| 关 键 词： | 糖尿病 胰岛素受体 巯基测定 |
Study on the Thiol Group of Insulin Receptor in Liver Membrane of Diabetic Rats |
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| GE Yi-li,SHENG Qing-fang,SONG Gong-wu.Study on the Thiol Group of Insulin Receptor in Liver Membrane of Diabetic Rats[J].Journal of Analytical Science,2011,27(2):227-230. |
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| Authors: | GE Yi-li SHENG Qing-fang SONG Gong-wu |
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| Institution: | GE Yi-li1,SHENG Qing-fang2,SONG Gong-wu1(1.School of Chemistry and Chemical Engineering,Hubei University,Wuhan 430062,2.HuBei Light Industry Technology Institute,Wuhan 430070) |
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| Abstract: | The diabetic rat model was set up by intraperitoneal injections of alloxan to Wistar rat.The property of thiol group was investigated by thiol group assay with ultraviolet-visible spectrophotometry.The experiment showed that the thiol group amounts of insulin receptor in liver membrane of rats were obviously decreased by 22% per gram protein(P<0.05).The results reveal that oxidative damage might exist in the insulin receptor of diabetic rats in liver membrane. |
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| Keywords: | Diabetes mellitus Insulin receptor Thiol group determination |
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