| 全自动在线固相萃取-高效液相色谱法测定水体中痕量微囊藻毒素 |
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| 引用本文： | 郭坚,杨新磊,叶明立.全自动在线固相萃取-高效液相色谱法测定水体中痕量微囊藻毒素[J].分析化学,2011,39(8):1256-1260. |
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| 作者姓名： | 郭坚 杨新磊 叶明立 |
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| 作者单位： | 1. 中英HUST-RRes作物基因工程和基因组学联合实验室,科技部国际科技合作基地,教育部分子生物物理重点实验室,武汉,430074;湖北出人境检验检疫局技术中心,武汉,430022
2. 戴安上海分析仪器有限公司,上海,201023 |
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| 摘 要： | 建立了全自动化在线固相萃取-高效液相色谱法快速、准确测定水体中3种痕量微囊藻毒素的新方法.样品用自动进样器连续注入固相萃取小柱完成富集后,通过双梯度高效液相系统中的一个泵(上样泵)实现净化,再通过阀切换将固相萃取小柱切换至分析流路中,用另一个泵(分析泵)将待测物冲洗至分析柱进行测定.方法使用Acclaim PA柱芯(1...
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| 关 键 词： | 在线固相萃取 微囊藻毒素 高效液相色谱 双梯度系统 |
Determination of Trace Level Microcystins in Water by Fully Automated Online Solid Phase Extraction Coupled with High Performance Liquid Chromatography |
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| GUO Jian,YANG Xin-Lei,YE Ming-Li.Determination of Trace Level Microcystins in Water by Fully Automated Online Solid Phase Extraction Coupled with High Performance Liquid Chromatography[J].Chinese Journal of Analytical Chemistry,2011,39(8):1256-1260. |
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| Authors: | GUO Jian YANG Xin-Lei YE Ming-Li |
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| Institution: | GUO Jian1,2,YANG Xin-Lei*3,YE Ming-Li3 1(China-UK HUST-RRes Genetic Engineering and Genomics Joint Laboratory,International Science & Technology Cooperation Base(Genetic Engineering) of Chinese Ministry of Science and Technology,the Key Laboratory of Molecular Biophysics of Chinese Ministry of Education,College of Life Science and Technology,Huazhong University of Science & Technology,Wuhan 430074) 2(Technology Center of Hubei Entry-Exit Inspection and Quarantine Bureau,Wuhan 430050) 3(Dionex Shanghai Anal... |
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| Abstract: | A fully automated online solid phase extraction(online SPE)-HPLC method was developed for the determination of three trace level microcystins(MCs) in water samples.The sample was first injected by autosampler to the online SPE column continuously followed by clean-up using loading pump with gradient program.Then the online SPE column was introduced to the analytical flow path by valve switching and the analytes were back-flushed to the analytical column using analytical pump for further determination.The Ac... |
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| Keywords: | Online solid phase extraction Microcystin High performance liquid chromatography Dual gradient system |
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