a Department of Chemistry, University of Science and Technology of China, Hefei 230026, China;b Department of Chemistry, Tsinghua University, Beijing 100084, China
Abstract:
Green fluorescent protein(GFP) plasmid was caged by 8-bromo-7-hydroxyquinolinyl chromophore(BHQ) for controlling its expression with exact spatiotemporal resolution.In vitro and in vivo experiments clearly verified that,comparing with Bhc caging, the expression level of caged GFP plasmid was dramatically decreased and then efficiently restored after subsequent photolysis.