|
|||||
|
|
Photoregulation of protein plasmid expression in vitro and in vivo using BHQ caging group |
|
| Authors: | Zhi Ping Zhanga Yi Ming Li Xiao Yun Chena Qing Xiang Guo a |
| Institution: | a Department of Chemistry, University of Science and Technology of China, Hefei 230026, China;b Department of Chemistry, Tsinghua University, Beijing 100084, China |
| Abstract: | Green fluorescent protein(GFP) plasmid was caged by 8-bromo-7-hydroxyquinolinyl chromophore(BHQ) for controlling its expression with exact spatiotemporal resolution.In vitro and in vivo experiments clearly verified that,comparing with Bhc caging, the expression level of caged GFP plasmid was dramatically decreased and then efficiently restored after subsequent photolysis. |
| Keywords: | Photoregulation Caging(uncaging) BHQ Green fluorescent protein plasmid Photolysis |
| 本文献已被 CNKI 等数据库收录！ | |
| 点击此处可从《中国化学快报》浏览原始摘要信息 | |
| 点击此处可从《中国化学快报》下载免费的PDF全文 | |