|
|||||
|
|
| 基于在位光还原银胶法的表面增强拉曼散射技术检测核酸碱基 | |
| 引用本文： | 吴雷,李海超,赵海峰,孙宇,许浩然,吕明,杨春花,李文钊,李正强. 基于在位光还原银胶法的表面增强拉曼散射技术检测核酸碱基[J]. 分析化学, 2011, 39(8): 1159-1165. DOI: 10.3724/SP.J.1096.2011.01159 |
| 作者姓名： | 吴雷 李海超 赵海峰 孙宇 许浩然 吕明 杨春花 李文钊 李正强 |
| 作者单位： | 1. 吉林大学分子酶学工程教育部重点实验室,长春,130012 2. 吉林大学生命科学学院,长春,130012 3. 吉林省长春市疾病预防控制中心,长春,130033 4. 长春医学高等专科学校,长春,130013 |
| 基金项目： | 国家自然科学基金面向项目(No.30870533)资助 |
| 摘 要： | 在环状有机物存在的条件下,Ag+经激光诱导可发生光还原反应,形成Ag原子聚集的银胶体.本研究通过拉曼散射光谱、紫外-可见光谱和扫描电镜证明,核酸碱基和Ag+混合溶液经过激光诱导可以生成银胶体颗粒.随着银胶体的生成,核酸碱基的拉曼光谱信号因为表面增强拉曼散射效应而得到明显增强.实验表明,本方法不但可以获得高信噪比的拉曼光... |
| 关 键 词： | 光还原反应 银胶体 表面增强拉曼散射 碱基检测 |
Detection of Nucleic Acid Bases by Surface Enhanced Raman Scattering Technique Based on in-situ Photo-reduced Silver Colloids |
|
| WU Lei,LI Hai-Chao,ZHAO Hai-Feng,SUN Yu,XU Hao-Ran,LU Ming,YANG Chun-Hua,LI Wen-Zhao,LI Zheng-Qiang. Detection of Nucleic Acid Bases by Surface Enhanced Raman Scattering Technique Based on in-situ Photo-reduced Silver Colloids[J]. Chinese Journal of Analytical Chemistry, 2011, 39(8): 1159-1165. DOI: 10.3724/SP.J.1096.2011.01159 | |
| Authors: | WU Lei LI Hai-Chao ZHAO Hai-Feng SUN Yu XU Hao-Ran LU Ming YANG Chun-Hua LI Wen-Zhao LI Zheng-Qiang |
| Affiliation: | WU Lei1,LI Hai-Chao2,ZHAO Hai-Feng1,SUN Yu2,XU Hao-Ran1,LU Ming1,YANG Chun-Hua4,LI Wen-Zhao1,LI Zheng-Qiang*1 1(Key Laboratory for Molecular Enzymology and Engineering,Ministry of Education,Jilin University,Changchun 130012) 2(College of life Science,Jilin Univisity,Changchun 130012) 3(Changchun City Illness Defend Control Center,Changchun 130033) 4(Changchun Medical College,Changchun 130013) |
| Abstract: | The formation of colloids induced by laser irradiation was proved by the means of Raman scattering,UV-Vis spectrum and transmission electron microscopy(TEM).The Raman signal of nucleic acid bases was slightly enlarged due to the surface-enhanced Raman scattering activity brought by the aggregation of silver atoms.Furthermore,the experiment results showed that the Raman spectra with high signal to noise ratio and with good repeatability could be obtained by this method and the Raman spectra of nucleic acid b... |
| Keywords: | Photo-reduction reaction Silver colloidal Surface enhanced Raman scattering Detection of nucleic acids bases |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |