| 15N-标记的气相色谱-同位素比质谱与气相气谱-质谱联用鉴定微生物反硝化作用 |
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| 引用本文： | 艾国民,郑海燕,张敏,刘志培. 15N-标记的气相色谱-同位素比质谱与气相气谱-质谱联用鉴定微生物反硝化作用[J]. 分析化学, 2011, 39(8): 1141-1146. DOI: 10.3724/SP.J.1096.2011.01141 |
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| 作者姓名： | 艾国民 郑海燕 张敏 刘志培 |
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| 作者单位： | 中国科学院微生物研究所,微生物资源前期开发国家重点实验室,北京,100101 |
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| 基金项目： | 中科院知识创新课题(No.KJCX2-YW-L08)资助项目 |
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| 摘 要： | 以好氧反硝化菌-产碱杆菌(Alcaligenes faecalis)在15N-KN03标记反硝化培养下所产气体与培养管中空气的混合气体为分析对象,在样品中N2/O2,CO2,N2O,H2O基线分离的基础上,利用气相色谱-同位素比质谱对混合气体中N2进行高精密度的δ15N分析,同时利用气相色谱-质谱联用的选择离子模式对混...
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| 关 键 词： | 气相色谱-同位素比质谱 气相色谱-质谱联用 反硝化作用 氮气 一氧化二氮 产碱杆菌 |
Isotopic Confirmation of Occurrence of Microbial Denitrification Based on N_2 and N_2O Production Monitored by Gas Chromatography/Isotope Ratio Mass Spectrometry and Gas Chromatography/Mass Spectrometry |
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| AI Guo-Min,ZHENG Hai-Yan,ZHANG Min,LIU Zhi-Pei. Isotopic Confirmation of Occurrence of Microbial Denitrification Based on N_2 and N_2O Production Monitored by Gas Chromatography/Isotope Ratio Mass Spectrometry and Gas Chromatography/Mass Spectrometry[J]. Chinese Journal of Analytical Chemistry, 2011, 39(8): 1141-1146. DOI: 10.3724/SP.J.1096.2011.01141 |
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| Authors: | AI Guo-Min ZHENG Hai-Yan ZHANG Min LIU Zhi-Pei |
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| Affiliation: | AI Guo-Min*,ZHENG Hai-Yan,ZHANG Min,LIU Zhi-Pei(State Key Laboratory of Microbial Resources,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101) |
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| Abstract: | A new 15N-labled procedure based on isotopic ratio monitoring of N2 by GC-isolink-IRMS(isotope artio mass spectrometer) with high precision and of N2O by GC-MS in SIM mode with high sensitivity was developed for the identification and confirmation of in vitro microbial denitrification.Gas metabolites,produced by Alcaligenes faecalis,including atmospheric gases in the confined cultivation tube,were analyzed using GS-CarbonPlot column,and a satisfactory baseline separation of N2/O2,CO2,N2O and water vapour wa... |
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| Keywords: | Gas chromatography-isotope ratio mass spectrometry Gas chromatography-mass spectrometry Denitrification Dinitrogen Nitrous oxide Alcaligenes faecalis |
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