| C18丙烯酰胺硅胶柱对肌肽分离能力的比较 |
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| 引用本文： | 霍萌,朱秋劲. C18丙烯酰胺硅胶柱对肌肽分离能力的比较[J]. 分析试验室, 2011, 30(5): 62-65 |
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| 作者姓名： | 霍萌 朱秋劲 |
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| 作者单位： | 贵州大学生命科学学院食品系;贵州大学食品科学工程研究中心; |
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| 基金项目： | 贵州省农业攻关项目(黔科合NY字(2009)3029号); 贵州大学博士基金项目(No.2007-036); 科技部科技人员服务企业行动项目(2009GJF20049)资助 |
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| 摘 要： | 建立了一种以牛肉中肌肽为代表,反相分离测定亲水性物质的方法.选用丙基酰胺键合硅胶亲水作用色谱柱,反相分析测定牛肉中亲水性成分-肌肽的含量,样品无需衍生处理.结合HPLC-MS联用技术确定了保留时间为10.276～10.609min的色谱峰就是肌肽峰.将该色谱柱与常规C18色谱柱进行对比后发现,该色谱柱对L-肌肽的保留能...
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| 关 键 词： | 丙基酰胺键合硅胶柱 肌肽 鉴定 含量测定 HPLC-MS |
Comparison between the C_(18) column and the venusil HILIC for separation of l-carnosine |
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| HUO Meng,ZHU Qiu-jin. Comparison between the C_(18) column and the venusil HILIC for separation of l-carnosine[J]. Chinese Journal of Analysis Laboratory, 2011, 30(5): 62-65 |
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| Authors: | HUO Meng ZHU Qiu-jin |
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| Affiliation: | HUO Meng1,2 and ZHU Qiu-jin1,2,3(1.Department of Food Science,College of Life Science,Guizhou University,Guiyang 550025,2.Food Science & Engineering Research Center,3.National Beef Processing Technology R&D Sub-centre,Huishui 550600) |
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| Abstract: | In present work,an efficient method for the content determination of L-carnosine in the beef by RP-HPLC was established using Venusil HILIC hydrophilic chromatographic column.This method is easy and no need for sample derivatization.It was combined with the HPLC-MS to identified the chromatographic peak of L-carnosine which the retention time was 10.276~10.609 min.Comparing the C18 chromatographic column with the Venusil HILIC,it was found that the seperation performance of L-carnosine by Venusil HILIC was ... |
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| Keywords: | Venusil HILIC L-carnosine Identification Content determination HPLC HPLC-MS |
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