| 提高维甲酸毛细管电泳分析灵敏度的微柱固相萃取法研究 |
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| 引用本文： | 张普敦,许国旺,熊建辉,郑育芳,石先哲,杨青,魏复盛.提高维甲酸毛细管电泳分析灵敏度的微柱固相萃取法研究[J].分析科学学报,2004,20(3):225-228. |
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| 作者姓名： | 张普敦 许国旺 熊建辉 郑育芳 石先哲 杨青 魏复盛 |
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| 作者单位： | 中国科学院大连化学物理研究所,国家色谱研究分析中心,辽宁,大连,116011;中国科学院大连化学物理研究所,国家色谱研究分析中心,辽宁,大连,116011;中国环境检测总站,北京,100029 |
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| 基金项目： | 国家自然科学基金(No.29975029) |
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| 摘 要： | 本文发展了一种适于复杂样品预处理的微柱固相萃取装置。该装置与毛细管电泳结合用于13-顺-维甲酸和全反式-维甲酸异构体的分析，灵敏度可提高100倍以上。维甲酸异构体的微柱固相萃取过程包括柱老化、上样、洗涤、洗脱以及柱再生等步骤。将该法用于加标血浆样品中的维甲酸分析，得到满意的结果。
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| 关 键 词： | 微柱固相萃取 毛细管电泳 维甲酸 灵敏度 |
| 文章编号： | 1006-6144(2004)03-0225-04 |
| 修稿时间： | 2002年11月18 |
Investigation on Improving the Sensitivity of Retinoic Acids by Capillary Electrophoresis and Micro-column Solid Phase Extraction |
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| ZHANG Pu-dun.Investigation on Improving the Sensitivity of Retinoic Acids by Capillary Electrophoresis and Micro-column Solid Phase Extraction[J].Journal of Analytical Science,2004,20(3):225-228. |
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| Authors: | ZHANG Pu-dun |
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| Institution: | ZHANG Pu-dun~ |
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| Abstract: | In this paper, a micro-column solid phase extraction (MicroSPE) setup was developed. The setup consisted of a manual pump, several insulin syringes and a teflon extraction catridge filled with solid phase. 13-cis-retinoic acid and all-trans-retinoic acid isomers were analyzed by capillary electrophoresis and the MicroSPE method. The sensitivity was improved more than 100-fold. The extraction procedure included conditioning, loading, washing, eluting and regenerating. The method was applied to the analysis of retinoic acid isomers in spiked plasma and the satisfactory result was obtained. |
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| Keywords: | Micro-column solid phase extraction Capillary electrophoresis Retinoic acid Sensitivity |
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