Potentiometric measurement of cell volume changes and intracellular ion concentrations under voltage-clamp conditions in invertebrate nerve cells |
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Authors: | Dierkes Paul Wilhelm Coulon Philippe Neumann Susanne Schlue Wolf-Rüdiger |
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Institution: | Institut für Neurobiologie, Heinrich-Heine-Universit?t Düsseldorf, Universit?tsstr. 1, 40225 Düsseldorf, Germany. dierkes@uni-duesseldorf.de |
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Abstract: | Because changes in cell volume might disturb the normal function of animal cells, most cells are endowed with volume-regulating mechanisms. Experimentally induced changes in cell volume are often paralleled by changes in the membrane potential, which might affect a variety of transport processes across the cell membrane and, in turn, volume regulation 1, 2]. We have shown previously that multi-barrelled ion-selective microelectrodes are useful tools for measurement of cell volume and the intracellular concentrations of ions that might be relevant to volume regulation 1]. To investigate whether voltage-dependent transport processes are involved in cell-volume regulation we combined the potentiometric technique of ion-selective microelectrodes with the voltage-clamp technique. This combination enables simultaneous recording of cell volume, relevant intracellular ion concentrations, and ion currents across the cell membrane at a fixed membrane potential. |
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