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A V-shaped cationic dye for nonlinear optical bioimaging
Authors:Maçôas Ermelinda  Marcelo Gema  Pinto Sandra  Cañeque Tatiana  Cuadro Ana M  Vaquero Juan J  Martinho José M G
Affiliation:Centro de Química-Física Molecular (CQFM) and Institute of Nanoscience and Nanotechnology (IN), Instituto Superior Técnico, 1049-001 Lisboa, Portugal. ermelinda.macoas@ist.utl.pt
Abstract:A symmetric cationic molecule with D-π-A(+)-π-D architecture was synthesized with high two-photon absorption cross-section (σ(2) ≈ 1140 GM). Application as a marker in fluorescence microscopy of living cells revealed its presence inside the cell staining vesicular shape organelles in the cytoplasm. Fluorescence lifetime imaging microscopy shows that it is also able to penetrate within the nucleus.
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