Production of Enantiopure Chiral Epoxides with E. coli Expressing Styrene Monooxygenase |
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| Authors: | Dominika Gyuranová ,Radka Š tadá niová ,Zuzana Hegyi,Ró bert Fischer,Martin Rebroš |
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| Affiliation: | 1.Faculty of Chemical and Food Technology, Institute of Biotechnology, Slovak University of Technology in Bratislava, Radlinského 9, 812 37 Bratislava, Slovakia; (D.G.); (Z.H.);2.Faculty of Chemical and Food Technology, Institute of Organic Chemistry, Catalysis and Petrochemistry, Slovak University of Technology in Bratislava, Radlinského 9, 812 37 Bratislava, Slovakia; (R.Š.); (R.F.) |
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| Abstract: | Styrene monooxygenases are a group of highly selective enzymes able to catalyse the epoxidation of alkenes to corresponding chiral epoxides in excellent enantiopurity. Chiral compounds containing oxirane ring or products of their hydrolysis represent key building blocks and precursors in organic synthesis in the pharmaceutical industry, and many of them are produced on an industrial scale. Two-component recombinant styrene monooxygenase (SMO) from Marinobacterium litorale was expressed as a fused protein (StyAL2StyB) in Escherichia coli BL21(DE3). By high cell density fermentation, 35 gDCW/L of biomass with overexpressed SMO was produced. SMO exhibited excellent stability, broad substrate specificity, and enantioselectivity, as it remained active for months and converted a group of alkenes to corresponding chiral epoxides in high enantiomeric excess (˃95–99% ee). Optically pure (S)-4-chlorostyrene oxide, (S)-allylbenzene oxide, (2R,5R)-1,2:5,6-diepoxyhexane, 2-(3-bromopropyl)oxirane, and (S)-4-(oxiran-2-yl)butan-1-ol were prepared by whole-cell SMO. |
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| Keywords: | styrene monooxygenase whole-cell biocatalysis chiral epoxides |
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