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Investigating natural rubber composition with Fourier Transform Infrared (FT-IR) spectroscopy: A rapid and non-destructive method to determine both protein and lipid contents simultaneously
Affiliation:1. CIRAD, UMR IATE, F-34060 Montpellier, France;2. Kasetsart Agricultural and Agro-Industrial Product Improvement Institute, Kasetsart University, Bangkok, Thailand;1. Department of Biomedical Engineering, Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia;2. Orthopedic Research Center, Faculty of Medicine, Mashhad University of Medical Science, Mashhad, Iran;3. Biomaterials National Cell Bank of Iran, Pasteur Institute, Tehran, Iran;4. Department of Biomedical Engineering, Amirkabir University of Technology, 15914 Tehran, Iran;1. College of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, China;2. Agricultural Product Processing Research Institute, Chinese Academy of Tropical Agriculture Sciences, Zhanjiang 524001, China;1. Department of Materials Science and Technology, Faculty of Engineering, Nagaoka University of Technology, 1603-1, Kamitomioka-machi, Nagaoka, Niigata, 940-2188, Japan;2. Department of Physical Chemistry, School of Chemical Engineering, Hanoi University of Science and Technology, No.1, Dai Co Viet Street, Hai Ba Trung District, Hanoi, Viet Nam;3. Department of Materials Science and Technology, Tokyo National College of Technology, 1220-2 Kunugida-machi, Hachioji, Tokyo, 193-0997, Japan;4. Department of Inorganic Chemistry, School of Chemical Engineering, Hanoi University of Science and Technology, No.1, Dai Co Viet Street, Hai Ba Trung District, Hanoi, Viet Nam;1. Department of Chemical Engineering, Universitat Politècnica de Catalunya Barcelona Tech, Terrassa, Spain;2. Department of Polymer Technology, Faculty of Chemistry, Gdansk University of Technology, Gdansk, Poland
Abstract:A large panel of Natural Rubber (NR) samples was characterized using Fourier Transform Infrared (FT-IR) spectroscopy in Attenuated Total Reflection (ATR) configuration. Specific vibrational bands were attributed to some non-isoprene compounds naturally present in NR composition. A rapid and non-destructive method was developed to investigate some specific functional groups contained in lipids (ester and carboxyl groups) and proteins (amides). Ester and carboxyl groups were quantified using calibration curves developed from synthetic cis-1,4-polyisoprene mixtures with either methyl stearate or stearic acid. Amide groups of proteins and peptides were found to be directly quantifiable from NR FT-IR spectra. The clonal origin and processing were found to influence the non-isoprene composition of NR. Significant correlations were found between FT-IR results and conventional chemical analyses: nitrogen content for proteins and total lipid extract.
Keywords:Natural rubber  FT-IR spectroscopy  Lipid  Protein
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