In-Vitro Cytotoxicity Study: Cell Viability and Cell Morphology of Carbon Nanofibrous Scaffold/Hydroxyapatite Nanocomposites |
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| Authors: | Asmaa M Abd El-Aziz Azza El-Maghraby Andrea Ewald Sherif H Kandil |
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| Institution: | 1.Fabrication Technology Research Department, Advanced Technology and New Materials Research Institute, City for Scientific Research and Technological Applications, Alexandria 23713, Egypt;2.Department of Materials Science, Institute of Graduate Studies and Research, Alexandria University, Alexandria 21526, Egypt;3.Department of Functional Materials in Medicine and Dentistry, University Hospital Würzburg Center for Dental, Würzburg University, 97070 Würzburg, Germany; |
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| Abstract: | Electrospun carbon nanofibers (CNFs), which were modified with hydroxyapatite, were fabricated to be used as a substrate for bone cell proliferation. The CNFs were derived from electrospun polyacrylonitrile (PAN) nanofibers after two steps of heat treatment: stabilization and carbonization. Carbon nanofibrous (CNF)/hydroxyapatite (HA) nanocomposites were prepared by two different methods; one of them being modification during electrospinning (CNF-8HA) and the second method being hydrothermal modification after carbonization (CNF-8HA; hydrothermally) to be used as a platform for bone tissue engineering. The biological investigations were performed using in-vitro cell counting, WST cell viability and cell morphology after three and seven days. L929 mouse fibroblasts were found to be more viable on the hydrothermally-modified CNF scaffolds than on the unmodified CNF scaffolds. The biological characterizations of the synthesized CNF/HA nanofibrous composites indicated higher capability of bone regeneration. |
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| Keywords: | HA modifiedCNF membranes cytotoxicity WST test cell counting cell viability cell morphology |
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