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Effect of Different Drying Methods on the Nutritional Value of Hibiscus sabdariffa Calyces as Revealed by NMR Metabolomics
Authors:Sengnolotha Marak  Elena Shumilina  Nutan Kaushik  Eva Falch  Alexander Dikiy
Institution:1.Amity Institute of Food Technology, Amity University Uttar Pradesh, Noida 201313, India;2.Amity Food and Agriculture Foundation, Amity University Uttar Pradesh, Noida 201313, India;3.Department of Biotechnology and Food Sciences, Norwegian University of Science and Technology (NTNU), 7491 Trondheim, Norway; (E.F.); (A.D.)
Abstract:Red mature calyces of Hibiscus sabdariffa were collected from 16 different locations in Meghalaya, India. Samples were processed using shade drying (SD) and tray drying (TD). NMR spectroscopy was used to assess the metabolic composition of the calyces. In this study, 18 polar metabolites were assigned using 1D and 2D NMR spectra, and 10 of them were quantified. Proximate analysis showed that the TD method is more efficient at reducing moisture and maintaining the ash content of the Hibiscus biomass. NMR metabolomics indicates that the metabolite composition significantly differs between SD and TD samples and is more stable in TD plant processing. The differences in post-harvest drying has a greater impact on the metabolite composition of Hibiscus than the plant location.
Keywords:Hibiscus  herbal processing  metabolic profiling  antioxidant  metabolites  NMR  Hibiscus acid
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