糖芯片的检测及应用

糖芯片技术具有样品少、通量高和特异性强等优点,是一种糖组学研究的新的技术平台和强大的分析工具,已经广泛用于糖和蛋白质的特异性作用、酶活性和抑制剂、病毒入侵机理、细菌检测和免疫反应等方面的研究.本文简要介绍了糖芯片的原理、制备和信号的检测技术(荧光标记法、质谱法、SPR法等),分析了糖芯片在各个领域的应用及其发展前景.

Detection and Application of Carbohydrate Microarrays

The biological significance of glycans in the post-genomic era requires the development of new technologies to enable functional studies of carbohydrates in a high-throughput manner.Recently,carbohydrate microarrays,as a new kind of promising biological-chip following the gene microarray and protein microarray have been exploited as an advanced technology for this purpose.It will highly promote the development of glycobiology due to the advantages of carbohydrate microarray such as less dosage of samples,hi...