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液体发酵云芝蛋白多糖的分离及其鉴定
引用本文:吴国荣,程光宇.液体发酵云芝蛋白多糖的分离及其鉴定[J].南京师大学报,1995,18(1):88-93.
作者姓名:吴国荣  程光宇
作者单位:南京师范大学生物系
基金项目:江苏省教育委员会自然科学基金
摘    要:放射诱变筛选出的高产云芝菌种用液体发酵法培养的菌丝体和发酵液经热水提取、乙醇沉淀、1万分子量截面超滤得到淡黄灰色粉末状云芝图丝体蛋白多糖和橙灰色状的发酵液蛋白多糖,菌丝体和发酵液蛋白多糖的糖含量,蛋白质含量分别为62.4%、23.5%,与43.8%、27.1%。与日本商品云芝蛋白多糖(PS-D(相比较,发酵云芝菌丝体蛋白多糖在红外、紫外吸收,物理常数、单糖和氨基酸的组成上是一致,后者在纯度上则高于

关 键 词:液体培养  多孔菌科  蛋白多糖  分离  云芝  发酵

Separation and Identification of the Protein-Polysaccharides from Fungus Polystictus Versicolor Hyphae Produced by Liguid Fermentation and it's Fermentative Syrup
Wu Guorong, Chen Guangyu, Chen Shenglan, Zou Yuzhen.Separation and Identification of the Protein-Polysaccharides from Fungus Polystictus Versicolor Hyphae Produced by Liguid Fermentation and it''s Fermentative Syrup[J].Journal of Nanjing Normal University(Natural Science Edition),1995,18(1):88-93.
Authors:Wu Guorong  Chen Guangyu  Chen Shenglan  Zou Yuzhen
Abstract:Hyphae of a high productivity mutant of fungus Polystictus versicolor which has been selected from radiation induction and incubated with liquid medium, and it's fermentative syrup, both extracted by hot water, precipitated by alcohole and ultratiltrated with cross section of 104 M. W., we obtained the yellowish gray powdery protein-polysaccha rides of fungus hyphae and the orange powdery protein-polysaccharides of fermentative syrup. The sugar and protein content of hyphae is 62.4% and 23.5%,and which of syrup is 43.8% and 27.1% respectively. Compave with Japan Product PS-K, according to the infraved and ultraviolet absorption spectrum, the physical constants, the component of monosaccharides and aminoacids, both are all identical, but the purity of our product is higher than PS-K. the monosaccharide component of these polysaccharides are mainly glucose.
Keywords:liquid culture  polyporaceael  proteoglycan  separation  discrimination  
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