Quantitative label-free RNA detection using surface-enhanced Raman spectroscopy |
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Authors: | Prado Enora Daugey Nicolas Plumet Sébastien Servant Laurent Lecomte Sophie |
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Affiliation: | Université Bordeaux 1-CNRS, Chimie et Biologie des Membranes et des Nanoobjets, 2 rue Robert Escarpit, 33607 Pessac, France. |
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Abstract: | Surface-Enhanced Raman Spectroscopy (SERS) was performed to detect label-free RNA. We defined conditions which make it possible to probe the four bases of RNA, in single strands of polyadenosine (pA), polyuridine (pU), polycytosine (pC) and polyguanosine (pG). We therefore present below a quantitative analysis of mixtures of non-hybridized single strands, based on the deconvolution of the SERS mixture spectrum into the relative contributions of the SERS spectra of each constituent. |
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