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水溶性发光金量子点灵敏检测L-半胱氨酸
引用本文:吴玉芹,陈金龙. 水溶性发光金量子点灵敏检测L-半胱氨酸[J]. 应用化学, 2013, 30(2): 225-231. DOI: 10.3724/SP.J.1095.2013.20127
作者姓名:吴玉芹  陈金龙
作者单位:(1.浙江建安检测研究院有限公司 杭州 310021;2.中国药科大学药学院 南京 210009;3.安庆师范学院化学化工学院 安庆 246001)
基金项目:安徽省教育厅基金资助项目,中国药科大学博士科研启动项目
摘    要:室温下一步合成了一种蓝色发光金量子点。 该金量子点具有良好的水溶性和生物相容性,金量子点平均粒径为3.0 nm,在波长305 nm光激发下,发射430 nm蓝色荧光。 实验发现,一定量L-半胱氨酸对金量子点430 nm处荧光发射有显著的增强作用,由此可建立一种简单、迅速、灵敏检测L-半胱氨酸的新方法。在最佳条件下,金量子点荧光强度与L-半胱氨酸在0~4.0 μmol/L浓度范围内呈线性关系,线性相关系数R2=0.9976,对2.0 μmol/L L-半胱氨酸的11次测定结果的相对标准偏差(RSD)为2.8%,以3倍标准偏差计算本法对L-半胱氨酸测定的检出限为5 nmol/L。

关 键 词:金量子点   荧光分析   半胱氨酸  生物传感器  
收稿时间:2012-03-31
修稿时间:2012-05-30

Sensitive Detection of L-Cysteine by Using Blue Luminescent Gold Quantum Dots
WU Yuqin , CHEN Jinlong. Sensitive Detection of L-Cysteine by Using Blue Luminescent Gold Quantum Dots[J]. Chinese Journal of Applied Chemistry, 2013, 30(2): 225-231. DOI: 10.3724/SP.J.1095.2013.20127
Authors:WU Yuqin    CHEN Jinlong
Affiliation:(1.Zhejiang Giian Test Institute Co.,Ltd,Hangzhou 310021,China;2.College of Pharmacy,China Pharmaceutical University,Nanjing 210009,China;3.College of Chemical and Chemical Eginerring,Anqing Normal University,Anqing 246001,China)
Abstract:Blue luminescent gold(Au) quantum dots(QDs) were facilely prepared through a one-pot synthesis at room temperature. The as prepared Au QDs were well water-soluble and biocompatable. The mean size of Au QDs is about 3.0 nm. Gold QDs colloids showed a considerable blue fluorescence emission at 430 nm under excitation of 305 nm. Experimental results demonstrated that the addition of L-cysteine could significantly enhance the characteristic emission of Au QDs at 430 nm. Therein, a simple, rapid, sensitive detection of L-cysteine in aqueous solution was established. Under optimized conditions, the characteristic fluorescence intensity of Au QDs at 430 nm is linearly proportional to the concentration of L-cysteine in the range of 0.0 to 4.0×10-6 μmol/L and a detection limit as low as 5 nmol/L was obtained. The relative linear relationship coefficient is 0.9976. The relative standard deviation for 11 replicate detections of 2.0 μmol/L L-cysteine was 2.8%. In addition, this method also demonstrated a high selectivity to amino acids due to the strong affinity of L-cysteine to gold atoms.
Keywords:Gold quantum dots  fluorescence  biosensor  L-cysteine
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